Answer to Question #43333 in Biochemistry for Sumit

Question

Chemical method to determine the number of polypeptide chains in a protein.

Answer 1

The chemical methodsare usually employed for amino acids analyses, end-group analysis, and quantitative analysisfor specific prosthetic groups. However, the presence of subunits can be established with the help of other methods: gel chromatography, sedimentation velocity, sedimentation equilibrium, and electrophoresis under nondenaturing conditions, followed by a second dimension under denaturing conditions