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A value of kLa = 30 h-1 has been determined for a fermenter at its maximum practical agitator rotational speed and with aie being sparged at 0.5 L gas / L reactor volume-min. E. coli with a qO2 of 10 mmol O2/g-dry wt-h are to be cultured. The critical dissolved oxygen concentration is 0.2 mg/L. The solubility of oxygen from air in the fermentation broth is 7.3 mg/L at 30°C. a) What is the maximum concentration of E. coli that can be sustained in this fermenter under aerobic conditions? b) What is the concentration could be maintained if pure oxygen was used to sparge the reactor?
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