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b. You collect the following data by monitoring the reaction at 400 nm for 1 min. Calculate the velocities in units of mol/min using Beer’s law (A=concentration*pathlength*molar absorptivity). Your pathlength is 1 cm and the molar absorptivity (ε) of p-nitrophenol at 400 nm is 17800/(M*cm). substrate (mM) Δabs/min Velocity (mol/min) 0.125 0.123 0.5 0.304 1 0.579 2 0.68 4 0.72 c. Create a Lineweaver-Burk plot with your data and turn it in. What are the Vmax and Km? d. You repeat your experiment, but this time adding a suspected inhibitor to your reaction. The equation of the Lineweaver Burk plot for this data is y = 6.5932x + 6.6064. What are the Vmax and, Km? What type of inhibitor is this and how do you know?
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