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In a study of the poisoning of fish, 0.500 g of samples of fish tissue were analysed for barium. Each sample was digested with a permanganate-sulfuric acid mixture, and the volume adjusted to 10 mL and to construct calibration curve, 0.500-g samples of uncontaminated fish tissue were injected with various amounts of Ba+2, as follows: Standard 0: 0.00µg Ba+2 Standard 1: 50 µg Ba+2 Standard 2: 100 µg Ba+2 Standard 3: 150 µg Ba+2 The absorbance values are indicated below: Standard Number 0; Absorbance 0.015 Standard Number 1; Absorbance 0.220 Standard Number 2; Absorbance 0.415 Standard Number 3; Absorbance 0.602 If all samples and standards were treated in identical manner, what was the concentration of barium (in µg Ba per g of fish) in a fish sample whose absorbance value was 0.421 and 0.378, respectively?
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