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{"ops":[{"insert":"You have access to a field site that contains soil contaminated with a gasoline spill. You work for a company that deals with clean-up of polluted sites. You decide that you want to try to use \u201cbioaugmentation\u201d \u2013 the addition of microbial cultures with suitable metabolic capabilities to a contaminated site in an effort to enhance degradation.\u00a0\ni)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Describe how you would set up an experiment that would allow you to obtain a microbial strain capable of degrading toluene, a component of gasoline.\u00a0\n\n\nii)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Mention the experimental set-up, the types of media used, experimental controls (if you think they might be needed).\n\n\niii)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0How you would confirm that the microorganism(s) that you obtain are indeed capable of degrading toluene?\n"}]}
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