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I) What are residues 83 and 170? II) Suggest a role for residues 83 and 170 in the enzyme mechanism? III) Comment on the protonation states of His-119 and His-176. What does it tell you about the pKa values of His-119 and His-176? IV) Describe the role of His-176 in the mechanism. Does the third image represent the end of the reaction? Why?
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