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UN2005/UN2401 ’18 Exam #3 Nov 15, 2018 1. You have a double stranded (ds) DNA molecule. You want to use PCR to make multiple copies of a section of it. Suppose you start with one molecule of ds DNA and do 5 cycles of PCR. A-1. How many different primers will you need? (1) (2) (3) (4) (5) (>5). A-2. How many molecules of each primer will you need? (1) (2) (3) (4) (5) (>5). A-3. Your primers are most likely to be (ss RNA) (ssDNA) (dsRNA) (dsRNA) (ss, but equally likely to be RNA or DNA). No explanation required for A-1 to A-3. Note: ds = double stranded; ss = single stranded. A-4. Consider two different primers. (There may or may not be more, but stick to 2.) The two primers are probably (complementary) (unrelated in sequence) (the same sequence but one is ‘backwards’).
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