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The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS. Combine the following: • 80g NaCl • 2g KCl • 14.4g Na2HPO4 (dibasic anhydrous) • 2.4g KH2PO4 (monobasic anhydrous) • 800mL distilled H2O
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