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Question 10: Why do you think the protocol does not say to dissolve compounds directly in 1 liter of water? Question 11: The PBS protocol above says to adjust pH to 7.4 with HCl. What does this imply on the pH of 10x PBS before adjusting the pH, would it be greater or smaller than 7.4? Question 12: The last step in the protocol is to autoclave the 10x PBS solution. Why do you think this step is important? Look up the definition of autoclave if you are unsure what it means. Question 13: Taking into account your response to question 5, now that you have made a 10x PBS solution, describe how you would prepare 1 liter of 1x working solution PBS, including which glassware you would use. Will you need to adjust the pH again?
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