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1. What are the ionic strengths of 1.0M of NaCl, (NH4)2SO4 and K3PO4? In which of these solutions would a protein be expected to be most soluble; least soluble? 2. In what order will the following amino acids be eluted from a column of P-cellulose ion exchange resin by a buffer at pH 6: arginine, aspartic acid, histidine and leucine? 3. The neurotransmitter γ-aminobutyric acid is thought to bind to specific receptor proteins in nerve tissue. Design a procedure for the partial purification of such a receptor protein. 4. A mixture of amino acids consisting of arginine, cysteine, glutamic acid, histidine, leucine, and serine is applied to a strip of paper and subjected to electrophoresis using a buffer at pH 7.5. What are the directions of migration of these amino acids and what are their relative mobilities?
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