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ATGGGCGTTACAGGAATATTGCAGTTACCTCGTGATCGATTCAAGAGGACATCATTCTTT CTTTGGGTAATTATCCTTTTCCAAAGAACATTTTCCATCCCACTTGGAGTCATCCACAAT AGCACATTACAGGTTAGTGCAATTGGTGGACAGGATGGAGACAATGGATACCGGCAGGTATTGGAGTTACAGGCGTTATAATTGCAGTTATCGCTTTATTCTGTATATGCAAATTTGTCTTT(TAG) • Restriction endonuclease sites: Nde I - 5’-CATATG-3’ Xho I - 5’-CTCGAG-3’ Q1.) According to the primer design rules, what is the sequence of the shortest forward primer that could be used to amplify the glycoprotein-encoding gene for cloning into a pET-vector for expression of a C-terminally hexahistidine-tagged recombinant glycoprotein protein in Escherichia coli? Please explain it step by step so I can understand better thank you.
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