The most common isotope of oxygen is oxygen-16 with a measured mass of 15.994915u.
The mass defect is "\\Delta m = 8m_p + 8m_n + 8m_e - m_{\\text{isotope}}" , because the atom consists of 8 protons, 8 neutrons and 8 electrons.
"\\Delta m = 8(m_p + m_n + m_e) - m_{\\text{isotope}}, \\\\\\Delta m = 8(1.00728 + 1.00866+0.00055) - 15.994915, \\\\\\Delta m = 0.137\\,\\mathrm{u} = 0.137\\cdot 1.6605\\cdot10^{-27} = 0.227\\cdot10^{-27}\\,\\text{kg}."
The binding energy is "\\Delta m c^2 = 0.227\\cdot10^{-27}\\cdot(3\\cdot10^8)^2 = 2\\cdot10^{-11}\\,\\mathrm{J}."
The binding energy per nucleon is "\\dfrac{\\Delta mc^2}{16} = 0.128\\cdot10^{-11}\\,\\mathrm{J}."
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