Question #60188
explain the use of visible light spectrophotometry in finding the concentration of solutions of coloured substances
Expert's answer
In chemical science spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.
Firstly UV spectrophotometer is calibrated by a range of artificially prepared solutions of a known concentration. The obtained responses are used to draw a graph of concentration-response dependence.
After that a solution with an unknown concentration of a component is tested on the UV spectrophotometer. The obtained response is than compared with the previously produced graph and the component concentration is recovered.
Firstly UV spectrophotometer is calibrated by a range of artificially prepared solutions of a known concentration. The obtained responses are used to draw a graph of concentration-response dependence.
After that a solution with an unknown concentration of a component is tested on the UV spectrophotometer. The obtained response is than compared with the previously produced graph and the component concentration is recovered.
