Answer to Question #136319 in Chemistry for rolly

Question #136319
How much NAD+ (mw=663g/mol) would I need to weigh to make up a 10nM solution of 1ml?
1
Expert's answer
2020-10-02T14:20:17-0400

The number of the moles nn of NAD+ contained in V=V = 1 mL of c=c= 10nM solution is:

n=cV=10109 (M)1103(L)=11011n=cV = 10·10^{-9}\text{ (M)}·1·10^{-3}\text{(L)} =1·10^{-11} mol.

Therefore, its mass will be:

m=nM=1011(mol)663(g/mol)=6.63109m = nM = 10^{-11}\text{(mol)}·663\text{(g/mol)} = 6.63·10^{-9} g.

As this mass is very small, one can advice to prepare a more concentrated solution from the available amount of NAD+ and then dilute it.


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