The number of the moles n of NAD+ contained in V= 1 mL of c= 10nM solution is:
n=cV=10⋅10−9 (M)⋅1⋅10−3(L)=1⋅10−11 mol.
Therefore, its mass will be:
m=nM=10−11(mol)⋅663(g/mol)=6.63⋅10−9 g.
As this mass is very small, one can advice to prepare a more concentrated solution from the available amount of NAD+ and then dilute it.
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