An enzyme binds its substrate with a Kd of 1uM. If the enzyme was present at a total concentration of 2nM and the substrate was present at a total concentration of 2uM, what fraction of the total enzyme would be bound to the substrate?
0.1 g of a substance dissolved in 22g of benzene lowers the freezing point of benzene by 0.2 degree celsius . calculate the molecular mass of the substances (Kf= 12 degree celsius mol
Nitrogen oxides (NOX) are a serious air pollutant produced by diesel engines.
Selective catalytic reduction (SCR) is a chemical process developed to solve
this problem by converting NOx to nitrogen in a reaction with urea. The
reaction below shows how urea reacts with nitrogen monoxide in this process.
4 NO(g) + 2 (NH2)2CO(s) + O2(g) 4 N2(g) + 2 CO2(g) + 4 H2O(g) + 1453 kJ
If the kinetic parameters of the uninhibited Cytochrome P450 3A4 are; KM = 2.36 µM and Vmax = 3.87 pM/min and the inhibition constant of fluconazole for Cytochrome P450 3A4 is KI = 9.21 µM, how much slower is the initial reaction rate of the break-down of Compound X (MW 300g/mol) (blood concentration: 6 µg/ml) by Cytochrome P450 3A4 in the presence of Fluconazole (blood concentration: 5 µg/ml)?