Answer to Question #318440 in Organic Chemistry for Quin

Question

 How would a defined media composition differ if the nitrogen source is ammonium (NH4) or ammonium nitrate (NH4NO3)? How much of each of these compounds would you add to1L  of media if you wanted to maintain 10:1:0.1 (C:N:P) ratio with glucose as the sole carbon source at 1g/L? (25 points)

Answer 1

Therefore they need special nutrient and growth environments. To grow these difficult microbes, microbiologists often turn to undefined media which is chosen based on price and more-so in this case by necessity as some microorganisms have never been cultured on defined mediaThese are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media..