Question

In what order would the following globular proteins emerge/eluted on gel filtration of a mixture on Sephadex G200? Ribonuclease (Mr=12,000), aldolase (Mr=159,000), hemoglobin (Mr=64,000), β-lactoglobulin (Mr=36,000), serum albumin (Mr=65,000).

Answer 1

aldolase (Mr=159,000), serum albumin (Mr=65,000), hemoglobin (Mr=64,000), β-lactoglobulin (Mr=36,000), Ribonuclease (Mr=12,000).