Question

. A peptide was cleaved into two smaller peptides with cyanogen bromide (CNBr) and into two different peptides by trypsin (Tryp). Their sequences were as follows: 

CNBr 1: Gly-Thr-Lys-Ala-Glu 

CNBr 2: Ser-Met 

Tryp 1: Ser-Met-Gly-Thr-Lys 

Tryp 2: Ala-Glu 

What was the sequence of the original peptide?

Answer 1

Tryp 1: Ser-Met-Gly-Thr-Lys