Answer to Question #254241 in Organic Chemistry for atenz

Question

In an enzyme reaction experiment, the concentration of the substrate S

S is varied and the velocity measured for each concentration, giving the following data.

[S](mM)= 0.2, 0.4, 0.6, 0.8, 1.0

V(μM/min)= 0.3, 0.49, 0.61, 0.7, 0.77

 

By sketching the Lineweaver-Burk plot of 1/V against 1/[S] determine an approximation of

Km and Vmax. No need to sketch.

 Which of the following is the best approximation of Km and the maximum velocity Vmax?

1) Km = 1.26 and Vmax = 2.5

2) Km = 1.25 and Vmax = 0.63

3) Km = 0.63 and Vmax = 1.25

4) Km = -0.63 and Vmax = 1.25

Answer 1

2) Km = 1.25 and Vmax = 0.63