Answer to Question #159485 in Organic Chemistry for Gom

Question

Hi!

I need help with a standardized method for determining synthetic organic dyes in food. And how it applies.

Thanks!

Answer 1

An example of a standardized method for determining synthetic organic dyes in food is by using a single cartridge for preconcentration and separation followed by photometric detection.

The method is based on preconcentration of the dyes using solid phase extraction on a cartridge filled with silica chemically modified with C16 groups from aqueous solution at pH 1 followed by elution with water/acetonitrile mixture containing 2 mmol·L^-1 KH₂PO₄ adjusted to pH 3 with a step gradient of acetonitrile content. This elution allows quantitative separation of the dyes which makes their individual spectrophotometric determination possible.