Question

How would I prepare 500 mL of 0.01 M phosphate buffer pH 7 from stock solutions of 0.2 M
NaH2PO4 and 0.2 M Na2HPO4?

Answer 1

Mix 48, 75 ml of 0,2 M monobasic sodium phosphate with 76,25 ml of 0.2 M diabasic sodium phosphate and dilute to a total of 500 ml with distilled water.