Answer to Question #52492 in Inorganic Chemistry for Alexis

Question

How much of each component would you need to make a 0.5 M sodium phosphate buffer containing 0.5 M KCl and 0.05% sodium azide, pH 8.0?

Answer 1

At first you need to prepare stock solutions of 1M NaH₂PO₄ and 1M of Na₂HPO4. Dissolve 138 g of NaH₂PO₄•H₂O in H₂O to final volume 1L. Dissolve 142 g of Na₂HPO₄ in water to final volume 1L.
Than mix 466 mL of 1M NaH₂PO₄ and 34 mL of 1M Na₂HPO₄.
Add 37 g of KCl, dissolve it.
Add 0.5 g of sodium azide.
Additionally check PH.
Add H₂O to 1L.