Question

Describe how you will determine concentration of tryptophan in grains of soy bean harvested from field fertilised with NPK 15-15-15 using UV/VIS spectroscopy

Answer 1

Using UV detection for amino acids in most cases requires using the absorption of the carboxyl group (-COOH) in the 200 to 210 nm range.

Tryptophan will absorbs more light at 280nm