Question

Six litres of 0.4M 3-(N-morpholino) propane sulfonic acid (MOPS) buffer of pH 7.8 are required. Available in the laboratory are 10M HCl and 10M NaOH and the basic form of MOPS (M.Wt = 210.3). The pKa for the MOPS zwitter ion is 7.2. Describe, showing the relevant calculations, the procedure for preparing the buffer

If 5ml of 10M HCl was added to 1 litre of the above buffer (question 9) what would be the resulting pH?

Answer 1

We should take 504.72 g of MOPS, 0.6 ul NaOH, and

5,495.28 H2O

Answer to question #9: pH=2.0792681