Six litres of 0.4M 3-(N-morpholino) propane sulfonic acid (MOPS) buffer of pH 7.8 are required. Available in the laboratory are 10M HCl and 10M NaOH and the basic form of MOPS (M.Wt = 210.3). The pKa for the MOPS zwitter ion is 7.2. Describe, showing the relevant calculations, the procedure for preparing the buffer
If 5ml of 10M HCl was added to 1 litre of the above buffer (question 9) what would be the resulting pH?
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