Initial concentration of resazurin is
"c=\\frac{m}{V}=\\frac{4.5\\cdot 10^{-4}g}{15.0 mL}=\\frac{0.45 mg}{15.0 mL}= 0.03 \\frac{mg}{mL}"
After each twofold dilution concentration decreases two times. Thus after four twofold dilutions concentration decreases 24=16 times.
Final concentration of resazurin is "\\frac{0.03\\frac{mg}{mL}}{16}=0.001875\\frac{mg}{mL}=1.875 \\times 10^{-3} \\frac{mg}{mL}"
Comments
Leave a comment