Answer to Question #266750 in General Chemistry for iyoss

Question

Problems on enzyme reaction kinetics

1. Initial rates of enzyme-catalyzed reaction for various substrate concentrations are listed below:

Rate

mol/(L min)x10^6 Substrate concentration

(mol/L)

177. 0.0041

173. 0.00095

125. 0.00052

106. 0.000103

80. 0.000049

67. 0.0000106

43 0.0000051

a) Evaluate Vmax and Km by a Lineweaver-Burk plot.

b) Using the Eadie-Hofstee plot, evaluate Vmax and Km.

c) Plotting reaction rate and substrate concentration, find another estimate of Vmax and Km.

Answer 1

177+173+125+106-(80+67+43)

= 391km