Answer to Question #239292 in General Chemistry for chen

Question

How to collect the product from the immobilized enzymes, in vitro?

Answer 1

Immobilized enzymes, which are enzymes attached onto solid particles, confer extra rigidity and stability to the three-dimensional structure of the protein and allow an easy separation of the biocatalyst. In this way, they facilitate the reutilization of the catalyst in various reaction cycles.

Immobilizing an enzyme allows for an increased resistance to variables such as temperature or pH. It also allows the enzymes to be stationary throughout the process, which makes it much easier for them to be separated and reused.

Immobilization makes the enzyme thermostable and prevents the loss of enzyme activity. The enzyme is immobilized in various methods such as absorption, entrapment, and covalent binding on various supports.