In separate test tubes, prepare 2mL of 0.2 M of the following buffer solutions by mixing volumes of 0.1 M salt and 0.1 M acid/base
Test tube Number
Buffer solution
Salt
Acid/Base
1
Acetate buffer
0.1 M CH3COOH
0.1 M CH3COOH
2
Phosphate buffer
0.1 M Na2HPO4
0.1 Na2HPO4
QUESTION:
The internal pH of a muscle is 6.8. Calculate the [H2PO4-]/[HPO42-] ratio in the cell. The second dissociation constant of phosphoric acid is 6.31x10-8
[H2PO4-]/[HPO42-] ratio=0.4
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