Question

1. Component A and B were successfully separated through one of the gas chromatographic techniques. The following data was obtained.            

 

      α= 1.03,    Ka = 2.3  L = 2.806 m    H = 2.415 mm 

     Calculate 

 

i) The plate number (N),  

ii) efficient plate number of A (Neff(A)) and B (Neff(B)) 

iii) resolution the separation (Rs) 

iv) Peak width of A (WbA) 

v) mobile uphold time (tM).

Answer 1

i)$N=16(tr/W)^2$

N, the number of theoretical plates, is one index used to determine the performance and effectiveness of columns

ii)$N= 2π(tr.H/A)^2$

Width is calculated from the peak area and height values. This method provides relatively accurate and reproducible widths, even for distorted peaks, but results in somewhat larger N values when peak overlap is significant.