Answer to Question #145150 in General Chemistry for Lana

Question

Borate buffer is used in gel electrophoresis. It is a combination of Boric Acid (MW 61.83 g/mol), titrated with NaOH to the desired pH.

Answer 1

a) Borate concentration (giving buffering capacity) can vary from 10 mM to 100 mM. As BBS is used to emulate physiological conditions (as in animal or human body), the pH value is slightly alkaline, ranging from 8.0–9.0..

b) 0.1 x 0.5 =0.05 mol.

0.05/0.2 = 0.25 L.= 250 ml borate buffer and 250 ml water.