Answer to Question #109798 in General Chemistry for Fagbariwo Esther

Question

How to separate the active components in dongorayo leaf

Answer 1

One of the methods:

The leaf samples are washed with water to remove dust and foreign particles. The leaves were separated from the stems and were dried at 40–45 °C for 5 days. After drying, the leaves were ground into a powder by using a grinder. The leaf powder sample was kept in an amber coloured bottle.

The powdered sample (140 g) was extracted with a methanol solvent (260 ml) by using a maceration method for 3 days. After extraction, the sample was filtered by using a Bruckner funnel. The methanol solvent was evaporated by using a rotary evaporator under reduced pressure at 20 °C for 1 h. The crude extract became a semi solid mass (11.15 g; 7.96%). The methanol semi solid mass (0.53 g) was transferred into a plastic tube for antioxidant activity, total phenols and biochemical screening tests.

Further the active components are separated by chromatography - TLC, HPLC, other methods... Resd here...https://www.sciencedirect.com/science/article/pii/S2405882316300667