Question

Absorption coefficient of a glycogen- losing complex is 70 at a length light of 450Lmolcm-1.what is the concentration when the transmission is 40% in a curette of 2cm.

Answer 1

It can be solved using Beer-Lambert Law. Therefore,

$log(I_0/I)=\epsilon lc$

where The Greek letter epsilon in these equations is called the molar absorptivity - or sometimes the molar absorption coefficient

l- the length of the light path in cm.

c-concentration of the solution

$logI_0-logI=\epsilon lc$

$0-log(0.4)=70*c*2$

$c=0.9948$