Biology Answers

A cross was made between pure breeding plants differing in two characters (colour and shape of seed) and the progenies were intercrossed to produce F2 population. Four types of phenotypes; yellow round (1200), yellow wrinkled (386), green round (372) and green wrinkled (144) were observed in F2. Use the chi-square test to determine if these results are due to independent assortment 

How do motility and secretions of the stomach contribute to the digestive process?

explain why mismatch repair is the more important for the accuracy of DNA Replication

If DNA of a particular species was analyzed and it was found that it contains 27 percent A, what would be the percentage of C?

Discuss gene expression with respect to energy efficiency to each mechanisms during transcription and translation

Difference between animal and plant celk

Discuss what happens when an individual with 17 chromosomes mates with an individual of the same species with 16 chromosomes.

You discovered that a species of bacteria can break down StyrofoamTM (polystyrene) products due to an

enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.

Polymerase Chain Reaction

After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR

using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer

briefly but completely.

a. DNA polymerase isolated from Thermus aquaticus

Answer: __________________________________

b. Deoxynucleotide triphosphates (dNTPs)

Answer: __________________________________

c.Forward and reverse primers

Answer:

You discovered that a species of bacteria can break down StyrofoamTM (polystyrene) products due to an

enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.

Task 1: DNA Extraction

To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of

the following procedures? Answer briefly but completely.

b. Adding RNase A and Proteinase K during extraction

Answer: __________________________________

c. Adding ethanol before recovering the DNA extract

Answer: __________________________________

Can Nonsense mutation be reversed by hydroxylamine? Why or why not?