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The genome of virus SV40 is a circular double‐stranded DNA molecule 5 kb long
(i.e., it contains 10,000 bases)
After many generations of growth in medium containing
14N, the medium was replaced with new medium containing
15N. After five generations, what is the ratio of light:heavy DNA strands?
(i.e., it contains 10,000 bases)
After many generations of growth in medium containing
14N, the medium was replaced with new medium containing
15N. After five generations, what is the ratio of light:heavy DNA strands?
The genome of virus SV40 is a circular double‐stranded DNA molecule 5 kb long
(i.e., it contains 10,000 bases)
A 1 kb region is amplified by PCR for four cycles. (Assume that specific
amplification of the target region occurs.) What fraction of the total DNA
does the target sequence constitute?
(i.e., it contains 10,000 bases)
A 1 kb region is amplified by PCR for four cycles. (Assume that specific
amplification of the target region occurs.) What fraction of the total DNA
does the target sequence constitute?
Assume that you are working with Drosophila and are studying genes, a, b, c, d, e,
and f that are all linked in a region on a chromosome, but their order is
unknown. In your work, you find that five deletions in the same region uncover
recessive alleles of the genes as follows:
Deletion 1 uncovers a, b, and c.
Deletion 2 uncovers a and c.
Deletion 3 uncovers a, c, and e.
Deletion 4 uncovers c, d, and e.
Deletion 5 uncovers d, e, and f.
a. What is the order of the genes?
b. Suppose you systematically attempt to generate a chromosomal deletion
(using X‐rays) that lacks all five genes, but you are unsuccessful. Suggest a
plausible explanation for this.
and f that are all linked in a region on a chromosome, but their order is
unknown. In your work, you find that five deletions in the same region uncover
recessive alleles of the genes as follows:
Deletion 1 uncovers a, b, and c.
Deletion 2 uncovers a and c.
Deletion 3 uncovers a, c, and e.
Deletion 4 uncovers c, d, and e.
Deletion 5 uncovers d, e, and f.
a. What is the order of the genes?
b. Suppose you systematically attempt to generate a chromosomal deletion
(using X‐rays) that lacks all five genes, but you are unsuccessful. Suggest a
plausible explanation for this.
Describe the sequence of events that occur when the virulent phage T4 infects
susceptible host cells.
susceptible host cells.
Can the lytic P1 phage infect the lysogen produced by integration of the
phage which has the gene order x1 x2 x3 att x4 x5
phage which has the gene order x1 x2 x3 att x4 x5
Can the lytic P1 phage infect the lysogen produced by integration of the
phage described in question a? Explain your answer. (Hint: The phage in
part a is Lambda.)
phage described in question a? Explain your answer. (Hint: The phage in
part a is Lambda.)
A temperate bacteriophage has the gene order x1 x2 x3 att x4 x5. What is the
gene order of the prophage
gene order of the prophage
Describe the expected result of a nonsense mutation in the gene for Mad1
Two strains of E. coli, A and B, are inoculated into a chemostat in equal
proportions. After one generation, the frequency of strain B is 20%. Calculate the
fitness of strain B relative to strain A.
proportions. After one generation, the frequency of strain B is 20%. Calculate the
fitness of strain B relative to strain A.
Disadvantage of MRI IS its inability to image
spinal cord
brain
bone
cancerous tissue
spinal cord
brain
bone
cancerous tissue
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#333702 on Dec 2022
#333702 on Dec 2022