Molecular Biology Answers

Why is the generation of single and double mutants were done using PCR-based QuikChnage site-directed mutagenesis?

How would you verify that the plasmid contains your insert of interest, in the proper position and orientation?

Describe how you would calculate ∆G0′ for the reaction: glucose + 6 O2 S6 CO2 + 6 H2O. If you were told that this reaction is highly exergonic, what would be the arithmetic sign (negative or positive) of the ∆G0′ you would expect for this reaction?

Questions 

Answer the following questions at the end of the report: 

1. Give ONE example each for commercial kits that can be used to extract i) genomic DNA from blood and ii) plasmid DNA from bacteria. Provide the full names of the kits and URLs to the products. 

2. Why vigorous mixing at step 4 of part C in the practical handout must be avoided. 

3. Besides agarose gel electrophohoresis, name another method to check the quantity and quality of purified DNA samples. 

C. Preparation of plasmid DNA from bacteria cell (E. Coli) – Alkaline method.

4. Add 200 µL of Alkaline lysis solution II (0.2 N NaOH, 1% SDS) to the suspension and mixed by inverting the tube 5 – 8 times until the suspension become clear and sticky.

In a laboratory lesson in histology, a student studied a micropreparation at a low magnification of the microscope, and then wanted to examine the structures of interest at a high magnification, but, despite attempts to focus the image, he did not achieve clarity, and the glass of the specimen broke. What mistakes were made when studying the micropreparation?

If you will construct several phylogenetic trees of mammalian species using protein sequences from cytochrome c, hemoglobin, and fibrinopeptides which trees would have a more or less similar tree topology? Which trees would look very different from each other? Why is this so?

: What makes ATP essential to the organelles and the cell itself?