Question

Explain in 5steps how an okazaki fragment is made

Answer 1

1. Initiation of replication: Double helix unwinds, providing single-stranded
DNA templates with the help of helicases and single-strand binding
proteins.
2. Synthesis of lagging strand:
- Priming for Okazaki fragment
by primase
- Elongation of fragment 100-200 bp by DDNA polymerase
-
Replacement of RNA primer by DNA by DNA polymerase
- Joining of fragments by
ligase