1. Initiation of replication: Double helix unwinds, providing single-stranded DNA templates with the help of helicases and single-strand binding proteins. 2. Synthesis of lagging strand: - Priming for Okazaki fragment by primase - Elongation of fragment 100-200 bp by DDNA polymerase - Replacement of RNA primer by DNA by DNA polymerase - Joining of fragments by ligase
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