Answer to Question #68209 in Molecular Biology for T

Question

Why would the following medium NOT be considered a chemically defined medium:
glucose, 5 grams (g); NH4Cl, 1g; KH2PO4, 1g; MgSO4, 0.3g; yeast extract, 5g;
distilled water, 1 litre

Answer 1

Answer:
Chemically defined medium is a growth medium suitable for the in-vitro cell culture of human or animal cells, in which all of the chemical components are known.
Medium with the provided composition will not be considered as a chemically stable one because yeast will consume some components and emit products of their metabolism. Since that, chemical composition of this medium will be changeable