Microbiology Answers

Kavalactones

1. Any other plants/organisms which have been found to contain this Kavalactone?
2. The names and structures of other natural products isolated from the plant/organisms and their relationship to the Kavalactones, if any!
3. Any more recent work on the Kavalactones and/or Kava plant?
4. A brief review of the isolation procedure, and any suggestions that might have for improving it.
5. What are the key features of the structural data which would tell you if you were dealing with this or a related to Kavalactones it is isolated from another plants.
6. Any structure-activity relationship (SAR) studies done on this Kavalactones.
7. Any synthetic work for which this Kavalactones wa sthe target.

When looking at DNA replication what are the most important steps and enzymes used ?

What is the overview of reproduction in Eumycota?

:

(1) a strain with a mutant gene encoding Pol I such that it no longer has polymerase activity (but retains both types of nuclease activities);                               

(2) a strain without RNaseH proteins;                                

(3) a strain with a mutant gene encoding Pol I such that it no longer has 5´ to 3´ exonuclease activities (but retains 3´ to 5´ nuclease and polymerase activities);

(4) a strain with the mutant Pol I described in (5)and a strain lacking all RNaseH proteins.

DNA polymerase I (Pol I) of E. coli consists of three functional parts (domains): an N-terminal domain with 5´ to 3´ exonuclease activities required for removal of the RNA primer, a central domain responsible for 3´ to 5´ exonuclease proofreading, and a C-terminal domain with polymerase activity. Pol I is thought to simultaneously remove RNA primers and fill in the gaps that result. A group of proteins known as RNaseH also have 5´ to 3´ exonuclease activity and can thus remove RNA primers. However, they lack the other two functions observed for Pol I. Predict the ability of the following mutants to replicate DNA:(1) a strain with a mutant gene encoding Pol I such that it no longer has polymerase activity (but retains both types of nuclease activities);   (2) a strain without RNaseH proteins;