Question

Results question regarding a serial dilution experiment testing the bacterial coliform content with a 10 fold from 10^-1 to 10^-6. Agar used was CLED, with peptone as a nutrient.

If results showed that the most concentrated form (10^-1) showed no bacteria presence after incubation. A good number colonies present (of two types) around a mid-line concentration (10^-3) and then little or no colonies on the least diluted (10^6), how can this be explained? Is this due to the nutrient competition between the bacteria?
Thank you in advance

Answer 1

These results can appear either due to the experimental error or due to the nutrient competition between the bacteria.