Answer to Question #170113 in Microbiology for Tayyibe Uğuz

Question #170113

How to make a standard curve for a qPCR on detection of the bacteria N Gonorrhea


1
Expert's answer
2021-03-10T08:09:08-0500

To perform a qPCR standard curve, you set up qPCR reactions to amplify different amounts of the same DNA sample.It generates the curve and calculates the efficiency of the reaction. Acceptable ranges are between 90 and 110% with a slope of the curve around -3.3 for an efficiency of 100%.


Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be the first!

Leave a comment

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS