Answer to Question #170113 in Microbiology for Tayyibe Uğuz

Question

How to make a standard curve for a qPCR on detection of the bacteria N Gonorrhea

Answer 1

To perform a qPCR standard curve, you set up qPCR reactions to amplify different amounts of the same DNA sample.It generates the curve and calculates the efficiency of the reaction. Acceptable ranges are between 90 and 110% with a slope of the curve around -3.3 for an efficiency of 100%.