Answer to Question #169637 in Microbiology for Cameron Hicks

Question

How do you determine the amount of 'Complete Media' needed for a cellular culture, is there formula?

Is it dependent on the cell type?

Answer 1

To get the right media for cell development depends on the type of cell. Microbial cultures need agar for growth plates or liquid system. Agar is always in liquid and powdered consistencies, available with additional nutrients for optimal growth. Use the trypan blue method for cell counting. To get a complete media for cell culture, we multiply the number of cells by the final volume of cells.