Question

Studies on bacteria capable of degrading antibiotics. Write down practical with apparatus and procedure.

Answer 1

The three fundamental mechanisms of antimicrobial resistance are (1) enzymatic degradation of antibacterial drugs, (2) alteration of bacterial proteins that are antimicrobial targets, and (3) changes in membrane permeability to antibiotics. Antibiotic resistance can be either plasmid mediated or maintained on the bacterial chromosome. The most important mechanism of resistance to the penicillins and cephalosporins is antibiotic hydrolysis mediated by the bacterial enzyme β-lactamase. The studies of resistance can be done through a sensitivity test using bacteria cultures and sensitivity discs.