Question

Use drawings to describe the process of cloning a fragment of human DNA, listing the steps and any important enzymes, molecules needed, and important features of these molecules involved. Include a description of how you could use a selectable marker to identify if bacteria have a plasmid. What could you include in your plasmid and on your plate to distinguish the bacteria that have recombinant DNA vs bacteria that have empty vector by color?

Answer 1

Isolation of donor DNA fragment or gene.

Selection of suitable vector.

Incorporation of donor DNA fragment into the vector.

Transformation of recombinant vector into a suitable host cell.

Isolation of recombinant host cell.