Answer to Question #113753 in Genetics for amna

Question

1.Select, copy and paste a region of DNA sequence from this CDKN2A gene with 800 bp containing the site of the mutation (let be in the middle).
2.How to AMPLIFY this 800 bp sequence and CONFIRM its expected size?
3.Explain the above AMPLIFICATION method in detail mentioning all steps and reaction ingredients.
4.Explain the above size CONFIRMATION method in detail mentioning all steps and reaction ingredients,to deduce the correct ORF from the mRNA sequence of the gene and underline the mutated amino acid.
*they are all for the same gene CDKN2A

Answer 1

AAAGATACCTGCTATATAATAAGTAATATACTTTTAAGTTTCCTTCAAAATATTCTTGGGAAGATGATAA
TAGGTACTGCTAATTCTATACCCAGTTAACATTTTGGAAACTAAGGTTGAAAATTGTGACTTAACTATAA
TTATGCATTAAATCTACAACACATCAAAGAATTTTGCATTTTGTACTCCTTACTAAGATCCAGTTTGAGT
AGGAAGATAAATTTTACAGTAATTCTGAATGAGGGAAGTTGGCACAGAGTTTCTAAAAGAGTACCTTCCT
TATAGCAAATACTAAATAATTGTGCTATATTGAATTTAATTAAATAGAGAATAGTAAAAGGGAGAAAGAA
ACATCCAATGTTTTGAAACTTCTAGAGATCTACTCCCAGGGACACATTGTTTTTTCTTAGCAAATCTGTT
TGGAGGTCTGCTCTACTTTCTCAGAGGTCTCCCTTTCATGCTGAAGCTATCTTTTTTCCTTGTGGAACAT
AAGTAATTAAATACCTTGCAATTATTTACCTAAGAAAGTGTTTCTTTCCCGTTTAAAATGCTCTTACCAC

1. DNA region of a gene (800 bp) - https://www.ncbi.nlm.nih.gov/nuccore/NG_007485.1?report=fasta