Answer to Question #73715 in Cell Biology for Jagadish kalita

Question

Describe the methods for separating macro molecules on the basis of
molecular size, charge, solubility and molecular affinity.

Answer 1

Separation based on molecular size can be performed with the help of gel filtration chromatography, or size-exclusion chromatography, when substances move through material containing cavities of certain size. Small molecules trap in these cavities and move slower, while the large ones move faster. For more information visit https://www.harvardapparatus.com/media/harvard/pdf/Guide+for+Gel+Filtration.pdf
Separation based on charge is performed with the help of ion exchange chromatography. Charged resin either attracts the particles or contributes to their faster passage through the tube. For more information visit https://www.biochemden.com/ion-exchange-chromatography/

Separation based on solubility is performed with the help of paper chromatography, when molecules move along with solvent according to their ability to interact with it. For more information see https://www.chemguide.co.uk/analysis/chromatography/paper.html
Separation by molecular affinity is performed with the help of affinity chromatography, when specific antibodies are used in the column. See for more information https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-affinity-purification.html