Answer to Question #299986 in Cell Biology for Assignment

Question #299986

After the centrifugation was over, he saw pellets at the bottom of the test tube. Do you think it should move on with his experiment after obtaining such result? Why or why not









1
Expert's answer
2022-02-22T11:31:01-0500

Yeah, it should move on with his experiment after obtaining such result because that is pellet looking for under the influence of gravitational force

Centrifugation is a method of separating molecules having different densities by spinning them in solution around an axis (in a centrifuge rotor) at high speed. It is one of the most useful and frequently employed techniques in the molecular biology laboratory. Centrifugation is used to collect cells, to precipitate DNA, to purify virus particles, and to distinguish subtle differences in the conformation of molecules. Most laboratoriesundertaking active research will have more than one type of centrifuge, each capable of using a variety of rotors. Small tabletop centrifuges can be used to pellet cells or to collect strands of DNA during ethanol precipitation. Ultracentrifuges can be used to band plasmid DNA in a cesium chloride gradient or to differentiate various structures of replicating DNA in a sucrose gradient.


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