Answer to Question #237800 in Cell Biology for Shelly

Question

 The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4.

 Combine the following:

·        80g NaCl

·        2g KCl

·        14.4g Na₂HPO₄ (dibasic anhydrous)

·        2.4g KH₂PO₄ (monobasic anhydrous)

·        800mL distilled H₂O

1.     Adjust pH to 7.4 with HCl

2.     Add H₂O to 1L

3.     Autoclave for 20 minutes on liquid cycle. Store at room temperature.

Which ions are being produced by this process,

Answer 1

Mixing the given reagents to prepare a phosphate-buffered saline (PBS) solution involves the following dissociation reactions in water: 

• NaCl(aq) ----------> Na⁺ (aq)+Cl^- (aq) 
• KCl(aq) ---------->K⁺(aq) + Cl⁻(aq) 
• Na₂HPO₄(aq) ----------> 2Na⁺ (aq) + HPO₂⁻⁴ (aq) 
• KH₂PO₄ (aq) ----------> K⁺ (aq) + H₂PO⁻⁴(aq) 
• HCl (aq) +H₂O (l) ----------> H₃O ⁺ (aq) + Cl ^- (aq) 

Based on the chemical equations above, we can see that the preparation process produces the following ions once the reagents are dissolved in water:  Na⁺ ,  K⁺ , Cl^- , HPO₂^-4 ,  H₂PO^-4 , H₃O⁺ .