The mitochondria is commonly known as the powerhouse of the cell as this is where ATP synthesis takes place. It is a membrane-bound organelle. It has an outer membrane and an inner membrane. The inner membrane forms cristae through its folding. There is fluid in between the folding and this space is called the matrix.
In the given question,
- Pyruvate decarboxylase is an enzyme that crosses the mitochondrial membrane to reach the matrix to perform its function.
- Succinate dehydrogenase is an enzyme that is involved in both the electron transport chain as well as the citric acid cycle. This enzyme is found in the inner mitochondrial membrane in eukaryotes and in many bacteria.
- Cytochrome complex is found loosely attached to the inner mitochondrial membrane. It is a hemeprotein that transfers electrons between complex III and complex IV.
- F0-F1 complex is present in the matrix of mitochondria. The F1 subunit functions as an ATPase when it detaches from the mitochondrial membrane.
From the above statements, it is clear that the only enzyme that is not attached to any mitochondrial membrane is pyruvate dehydrogenase. Hence, the option is (1) pyruvate dehydrogenase.
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