Answer to Question #171299 in Cell Biology for Cameron Hicks

Question #171299

I am looking at studying depolarization in catfish, using a cell culture I hope to measure this using muscle cells taken from the swimming muscle. I am also looking into the effect of a cholinergic crisis on depolarization. My question is, would I be able to sustain these cells under a cholinergic crisis, if I provided the necessary nutrients (Could this be explained if not)? If not, what causes damage to the depolarization function of the muscle cell during this crisis?


Many thanks


1
Expert's answer
2021-03-15T07:35:09-0400

Yes,

Depolarization of cone catfish horizontal cells induces an extracellular. If a stimulus reaches or exceeds the threshold potential of the cardiac muscle cell (about -70 mV), phase 0 takes place. This is known as the depolarization period and is caused by the opening of the sodium voltage-gated time-dependent channels and this allows sodium ions into the cell.


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