Question #145556
Consider the following pair of wild-type and mutant template strand sequences.
Wild type: 5’ CCAGTATGGCAT 3’
Mutant: 5’ CCAGTACTGGCAT 3’
What are the amino acid sequences coded by both strands? What type of mutation has occurred?
Wild type: 5’ CCAGTATGGCAT 3’
Mutant: 5’ CCAGTACTGGCAT 3’
What are the amino acid sequences coded by both strands? What type of mutation has occurred?
Expert's answer
Wild type: 5’ CCA GTA TGG CAT 3’
RNA m: 3’ GGU CAU ACC GUA 5’
RNA t: ССА GUA UGG CAU
Amino acid: Pro – Val – Trp – His
Mutant: 5’ CCA GTA CTG GCA T 3’
RNA m: 3’ GGU CAU GAC CGU A 5’
RNA t: ССА GUA CUG GCA
Amino acid: Pro – Val – Leu – Ala
There was an insertion of the C nucleotide into the DNA sequence between the 6 and 7 nucleotides [6-7ins C]. This led to a shift in the reading frame and the replacement of the two amino acids Trp with Leu and His with Ala.
